不同品种鸡对肠炎沙门氏菌人工感染初期免疫应答反应的差异

doi:10.11843/j.issn.0366-6964.2015.06.005

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (6): 903-910.doi: 10.11843/j.issn.0366-6964.2015.06.005

不同品种鸡对肠炎沙门氏菌人工感染初期免疫应答反应的差异

刘璐^1，2，李建超^1，2，刘冉冉²，李庆贺²，文杰²，常国斌¹，郑麦青²，陈国宏^1*，赵桂苹^2*

（1.扬州大学动物科学与技术学院，扬州 225009；2.中国农业科学院北京畜牧兽医研究所，北京 100193）

收稿日期:2014-11-07 出版日期:2015-06-23 发布日期:2015-06-23
通讯作者: 赵桂苹，研究员，主要从事家禽遗传育种研究，Tel：010-62816019，E-mail：zhgp0402@iascaas.net.cn；陈国宏，教授，主要从事家禽遗传育种研究，Tel：0514-87979206，E-mail：ghchen@yzu.edu.cn
作者简介:刘璐（1990-），女，江苏徐州人，硕士，主要从事家禽遗传育种研究，E-mail：m13051343248@163.com。刘璐和李建超同为第一作者
基金资助:
国家现代农业肉鸡产业技术体系（CARS-42）；江苏省农业科技支撑计划（BE2013392）

Differences of Early Innate Immunity between Chicken Breeds against Salmonella Enteritidis Infection

LIU Lu^1，2，LI Jian-chao^1，2，LIU Ran-ran²，LI Qing-he²，WEN Jie²，CHANG Guo-bin¹，ZHENG Mai-qing²，CHEN Guo-hong^1* ，ZHAO Gui-ping^2*

(1.College of Animal Science and Technology，Yangzhou University，Yangzhou 225009，China；2.Institute of Animal Science，Chinese Academy of Agricultural Sciences，Beijing 100193，China)

Received:2014-11-07 Online:2015-06-23 Published:2015-06-23

摘要/Abstract

摘要：

本试验以中国地方鸡种北京油鸡（Beijing-You chicken，BJY）和引入蛋鸡品种白来航鸡（White Leghorn，WL）为试验对象，通过人工感染肠炎沙门氏菌（Salmonella Enteritidis，SE），研究两个不同遗传背景新生雏鸡在接种沙门氏菌后，部分免疫指标和相关基因的变化规律，揭示两品种鸡在早期对SE感染抗性的的异同点。选择1日龄的北京油鸡和白来航鸡各40只，隔离舱饲养，相同日粮水平条件下，两侧胸肌各注射8.7×10⁸ cfu•mL^-1沙门氏菌0.5 mL，分别在攻毒后12、24、72 和144 h（12 hpi、24 hpi、72 hpi、144 hpi）测定IgY、IL-6和TNF-α血清介质变化，荧光定量检测血液载菌量、盲肠组织中TLR4受体、DNMT3a、DNMT3b和DNMT1甲基转移酶基因的表达情况。结果表明：（1）攻毒沙门氏菌后，北京油鸡的IgY、IL-6、TNF-α血清介质水平显著高于白来航鸡（P<0.05）；（2）在12 hpi、24 hpi和72 hpi，血液载菌量品种间差异不显著（P>0.05），但在144 hpi白来航鸡血液中载菌量显著高于北京油鸡（P<0.05）；（3）在24 hpi， TLR4受体在北京油鸡盲肠组织中的表达显著高于白来航鸡；北京油鸡与白来航鸡DNMT3a、DNMT3b和DNMT1甲基转移酶基因在两品种间的表达差异显著（P<0.05）。结果提示，北京油鸡和白来航鸡对沙门氏菌的免疫性状具有明显的遗传差异，北京油鸡对沙门氏菌的抗性优于白来航鸡。

Abstract:

This study was conducted to study the effect of S.Enteritidis infection on early immune performance between two chicken breeds(the Beijing-You chicken(BJY)，a Chinese indigenous breed，and the White Leghorn(WL)，an imported laying type breed).40 BJY chickens and 40 WL chickens on 1-day-old were reared in isolators during the same period and were challenged by intramuscular injection with 8.7×10⁸ cfu•mL^-1 per chick(0.5 mL) of S.Enteritidis suspension，the concentrations of cytokines，antibodies and the S.Enteritidis colonization level in the blood and the expression of TLR4 and methyltransferase genes were determined in each group at 12，24，72 and 144 h after post-infection (12 hpi，24 hpi，72 hpi，144 hpi).The results showed that：（1）After challenged by S.Enteritidis，the two breeds showed significant differences in the serum antibody levels of IgY，IL-6，TNF-α，the antibody levels in BJY were higher(P<0.05)；（2） The SE colonization level in the blood of BJY was significantly lower than that of WL at 144 hpi (P<0.05)，while there were no differences at 12 hpi，24 hpi，72 hpi between two breeds(P>0.05)；（3） At 12 hpi the relative quantity of TLR4 in caecum in WL chickens was higher than that in BJY chickens，while at 24 hpi BJY chickens was significantly higher than that in WL chickens(P<0.05).There were significant differences for methyltransferase genes (DNMT1，DNMT3A and DNMT3B) expression in caecum between the two breeds(P<0.05).The results demonstrated that the two breeds differed markedly in immune traits after S.Enteritidis infection.BJY chicken was superior than WL chickens in considering of immune index.

中图分类号:

S831
S813.3

刘璐，李建超，刘冉冉，李庆贺，文杰，常国斌，郑麦青，陈国宏，赵桂苹. 不同品种鸡对肠炎沙门氏菌人工感染初期免疫应答反应的差异[J]. 畜牧兽医学报, 2015, 46(6): 903-910.

LIU Lu，LI Jian-chao，LIU Ran-ran，LI Qing-he，WEN Jie，CHANG Guo-bin，ZHENG Mai-qing，CHEN Guo-hong，ZHAO Gui-ping. Differences of Early Innate Immunity between Chicken Breeds against Salmonella Enteritidis Infection[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 903-910.

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不同品种鸡对肠炎沙门氏菌人工感染初期免疫应答反应的差异

Differences of Early Innate Immunity between Chicken Breeds against Salmonella Enteritidis Infection

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